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doxycycline inducible aavs1 integrated taup301l 2n4r egfp  (Addgene inc)


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    Structured Review

    Addgene inc doxycycline inducible aavs1 integrated taup301l 2n4r egfp
    Doxycycline Inducible Aavs1 Integrated Taup301l 2n4r Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cag+rtta3/AAVS1+CAG+rtTA3+TauP301L+2N4R-EGFP+(Plasmid+%23132393)/pm42902961-89-19-22
    Average 93 stars, based on 4 article reviews
    doxycycline inducible aavs1 integrated taup301l 2n4r egfp - by Bioz Stars, 2026-11
    93/100 stars

    Images

    Related Articles

    Virus:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    Polymerase Chain Reaction:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    Recombinant:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    Selection:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    Transfection:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    Knock-Out:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    Cloning:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    Plasmid Preparation:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    PCR Cloning:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    cDNA Synthesis:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    DNA Sequencing:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    Software:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    Digital PCR:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    Imaging:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).

    Flow Cytometry:

    Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
    Article Snippet: Components that were used in multiple constructs (e.g. pCAG and pA signal) were also cloned into a pSMART vector backbone using the CloneSmart HCKan Cloning Kit (Lucigen).



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    Image Search Results


    Oleocanthal (OLC) prevents P301L tau peptide aggregate‐induced phosphorylation and oligomerization. (a) Phase contrast and fluorescence microscopy images of differentiated SY5Y‐TauP301L‐EGFP cells showing the distribution of Tau (P301L) (green) following induction with doxycycline and nuclear staining (Hoechst, blue). Scale bar: 100 μm. (b) Western blot analysis of insoluble fractions (pSer262, T22, total tau) from cells transduced with R2R3 (P301L) tau peptide aggregated in the absence (control) or presence of OLC. GAPDH from soluble fractions was used as a loading control. Buffer: Cells transduced with the reaction mixture without the aggregation reaction product. Arrows indicate the position of TauP301L‐EGFP, expressed from the transfected plasmid, whereas brackets indicate endogenous wild‐type (WT) tau. Full‐length images of blots are shown in Figure . (c) Quantification of pSer262, T22, and total tau in the insoluble fraction. Signals were normalized to GAPDH; pSer262 and T22 were additionally normalized to total tau and shown as fold change vs. buffer control. Mean ± SEM ( n = 3). ** p < 0.01, * p < 0.05, one‐way ANOVA (Tukey's post hoc test).

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Structural and functional insights into the selective inhibition of mutant tau aggregation by purpurin and oleocanthal in frontotemporal dementia

    doi: 10.1002/pro.70240

    Figure Lengend Snippet: Oleocanthal (OLC) prevents P301L tau peptide aggregate‐induced phosphorylation and oligomerization. (a) Phase contrast and fluorescence microscopy images of differentiated SY5Y‐TauP301L‐EGFP cells showing the distribution of Tau (P301L) (green) following induction with doxycycline and nuclear staining (Hoechst, blue). Scale bar: 100 μm. (b) Western blot analysis of insoluble fractions (pSer262, T22, total tau) from cells transduced with R2R3 (P301L) tau peptide aggregated in the absence (control) or presence of OLC. GAPDH from soluble fractions was used as a loading control. Buffer: Cells transduced with the reaction mixture without the aggregation reaction product. Arrows indicate the position of TauP301L‐EGFP, expressed from the transfected plasmid, whereas brackets indicate endogenous wild‐type (WT) tau. Full‐length images of blots are shown in Figure . (c) Quantification of pSer262, T22, and total tau in the insoluble fraction. Signals were normalized to GAPDH; pSer262 and T22 were additionally normalized to total tau and shown as fold change vs. buffer control. Mean ± SEM ( n = 3). ** p < 0.01, * p < 0.05, one‐way ANOVA (Tukey's post hoc test).

    Article Snippet: AAVS1 CAG rtTA3 TauP301L 2N4R‐EGFP was a gift from Gerold Schmitt‐Ulms (Addgene plasmid #132393; http://n2t.net/addgene:132393 ; RRID: Addgene_132393).

    Techniques: Phospho-proteomics, Fluorescence, Microscopy, Staining, Western Blot, Transduction, Control, Transfection, Plasmid Preparation